The Scotia Rapid Test for Amnesic Shellfish Poisoning (ASP)

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The SRT ASP test is a qualitative lateral flow rapid test for ASP toxins that can be used with bivalves such as mussels, clams and oysters.  This test involves converting the shellfish into a liquid form (known as the extract) which can then be applied to the test.

This test is used as a screen to reduce the volume of samples being tested by analytical methods. A negative sample will require no further testing. A positive sample should be analyzed by a quantitative method. This screening protocol can significantly reduce the costs associated with analytical testing by government regulators.

The testing method is completed in three basic steps:

 

Step 1: Prepare sample   

For Shellfish: Remove tissue from the shell and grind till smooth

For Algae: Filter seawater and collect algal cells

 

Step 2: Prepare Extract   

For Shellfish: Extraction process converts ground shellfish into a liquid form using one of three methods:

1. Positive results obtained in a regulated environment will need further analytical testing by High Performance Liquid Chromatography (HPLC). This will require using the AOAC Extraction.

2. Positive results obtained in a regulated environment will need further analytical testing by Liquid Chromatography Mass Spectrometry (LCMS).  This will require using the Methanol Extraction

3. If further testing is not required, then use Rapid Extraction.

 

For Algae:

Extraction process converts the collected algal cells to liquid form using the Phyto Extraction.

 

Step 3: Proceed to Scotia ASP Test

To test, dilute shellfish or algal extract in the buffer provided with kit and apply sample to the test cassette.

For further details on how to perform the test see ASP AOAC , ASP Methanol , ASP Rapid  or ASP Phyto testing instructions.

 

 

 

 

 

 

 

 

Example of negative and positive (toxins present) test results:

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Please see kit instructions for complete test interpretation.

 

 

 

 

 

Pricing and ordering

 

 

Product Specifications
 

Sensitivity in shellfish

8-10 ppm (half of regulatory limit)

Sensitivity in phytoplankton

0.05-0.08 µg/mL

Test Time

30-45 minutes

Storage

4-25°C

Shelf Life

1 year

Usage

Single sample use

 

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