The Scotia Rapid Test for Paralytic Shellfish Poisoning (PSP)

DSP test kit sample

The SRT PSP test is a qualitative lateral flow rapid test for PSP toxins that can be used with bivalves such as mussels, clams and oysters.  This test involves converting the shellfish into a liquid form (known as the extract) which can then be applied to the test.

This test is used as a screen to reduce the volume of samples being tested by analytical methods. A negative sample will require no further testing. A positive sample should be analyzed by a quantitative method. This screening protocol can significantly reduce the costs associated with analytical testing by government regulators.

The SRT PSP test has regulatory approval  by the USFDA ISSC for use as a screen using the AOAC extraction method.

 

The testing method is completed in three basic steps:

Step 1: Prepare sample   

For Shellfish: Remove tissue from the shell and grind till smooth

For Lobster Tomalley: Remove tomalley from the lobster and blend till smooth

For Algae: Filter seawater and collect algal cells

 

Step 2: Prepare Extract   

For Shellfish: Extraction process converts ground shellfish into a liquid form using one of two methods:

 

1. Positive results obtained in a regulated environment will need further analytical testing by High Performance Liquid Chromatography (HPLC) or Mouse Bioassay (MBA). This will require using the AOAC Extraction.

2. If further testing is not required, then use Rapid Extraction.

 

For Lobster Tomalley:

Extraction process converts blended tomalley into a liquid form. This will require AOAC Extraction method.

 

For Algae:

Extraction process converts the collected algal cells to liquid form using the Phyto Extraction.

 

Step 3: Proceed to Scotia PSP Test

To test, dilute shellfish, tomalley or algal extract in the buffer provided with kit and apply sample to the test cassette.

For further details on how to perform the test see PSP AOAC, PSP Rapid or PSP Phyto testing instructions.

 

 

 

 

 

 

 

 

Example of negative and positive (toxins present) test results:

http://www.jellett.ca/admin/uploader/uploaded/size_actual/upload43921463493658.jpg

Please see kit instructions for complete test interpretation.

 

 

 

 

Pricing and ordering

 

Product Specifications
 

Sensitivity in shellfish

20-70 µg/100 g

Sensitivity in phytoplankton

0.1 µg/mL

Test Time

35 minutes

Storage

4-25°C

Shelf Life

1 year

Usage

Single sample use

 

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